April 2025 Newsletter

March 31, 2025

By Evolene Premillieu

Contents

3D images of different organoids from the Bruker.
Data acquired with the Bruker TruLive3D. Top left: organoids, image courtesy of Nolan Petrich. Top right: slice view of organoids, image courtesy of Nolan Petrich, scale bar is 50um. Bottom left: single cells, image courtesy of Dr Alex Kang. Bottom right: organoid, image courtesy of Dr Kaustav Bera. Scale bar is 100um for the volume images.

Bruker TruLive3D Light Sheet Microscope update

The Beckman Center Light Sheet Specialist is delighted to share some exciting news about our two specialized Light Sheet Microscopes.

The Bruker TruLive3D Light Sheet Microscope was purchased in 2023 by the Beckman Center and was first made available to targeted groups only. We have the pleasure to announce that it is now accessible to anyone on campus and beyond and is part of the ALMC booking system. Proceed as usual with our ticketing system to request a training on it. The Light-Sheet specialist will always be present with you during your imaging time to assist and help you with all your needs.

Several users recently got amazing results during multi-days, environment-controlled experiments. We will be show-casing their work on a portfolio page on our Wiki.

The Bruker TruLive3D is specifically designed to image organoids, spheroids, small embryos and single cells. Acquiring multiple z-stack is a breeze with this novel instrument and gives you direct 3D imaging. Each multi-days experiment will produce a time-lapse of your sample growing, ready to analyze with our Imaris workstation.

Schematic of a light sheet microscope.
Working principle of the Bruker TruLive3D Light Sheet Microscope. The custom sample holders are provided by the Beckman Center.

Single Objective Light Sheet Microscope

The second light sheet microscope of the Beckman Center is a Single Objective Light Sheet Microscope. This original, custom design microscope is ideal for single cells, small organoids or any first 1-3 layers of cells of a sample. The first prototype is now operational for a limited number of users but will gradually be made accessible for a wider audience so stay tuned!

3D view of Actin cells.
Data acquired with the Single Objective Light Sheet. 3D view of Actin cells. Scale bar is 50um.
Picture of a light sheet microscope.
Original custom-built Single Objective Light-Sheet Microscope.

Recent publications